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Date: 29 May 2006 19:35:31
From: nickstrachan
Subject: yeast culturing "Streaking"
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Does anyone have a link to pictures of yeast culturing partuicularly
the streaking method on petri dishes or plates....Right now im reading
an article upon the subject but i cant seem to picture it....
>Stab the loop in clean agar, flick it through the just-streaked area two or three times, and >streak a small area of fresh agar. When done, there should be a rectangular patch of clear >agar extending to the center of the plate, with the stab-marks at the bottom.
It goes onto one more step but i cant get my mind around this
process...Guess im more of a hands on show type..so if anyone had any
pics of the process i'd be much obliged.
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Date: 29 May 2006 23:50:28
From: JS
Subject: Re: yeast culturing "Streaking"
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On 29 May 2006 19:35:31 -0700, "nickstrachan" <nickajstrachan@aol.com >
wrote:
>Does anyone have a link to pictures of yeast culturing partuicularly
>the streaking method on petri dishes or plates....Right now im reading
>an article upon the subject but i cant seem to picture it....
>
>>Stab the loop in clean agar, flick it through the just-streaked area two or three times, and >streak a small area of fresh agar. When done, there should be a rectangular patch of clear >agar extending to the center of the plate, with the stab-marks at the bottom.
>
>
>It goes onto one more step but i cant get my mind around this
>process...Guess im more of a hands on show type..so if anyone had any
>pics of the process i'd be much obliged.
I recommend the booklet, The Fungus Among Us. Believe I got it from
Morebeer.com. Has drawings and instructions, plus how to maintain a
sanitary environment during the process. Good basic info without
getting techo-geeky.
John S.
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Date: 30 May 2006 21:23:31
From: nickstrachan
Subject: Re: yeast culturing "Streaking"
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rb wrote:
> nickstrachan wrote:
> > Does anyone have a link to pictures of yeast culturing partuicularly
> > the streaking method on petri dishes or plates....Right now im reading
> > an article upon the subject but i cant seem to picture it....
> >
> >> Stab the loop in clean agar, flick it through the just-streaked area two or three times, and >streak a small area of fresh agar. When done, there should be a rectangular patch of clear >agar extending to the center of the plate, with the stab-marks at the bottom.
> >
> >
> > It goes onto one more step but i cant get my mind around this
> > process...Guess im more of a hands on show type..so if anyone had any
> > pics of the process i'd be much obliged.
> >
> This works for bacteria, though the principle should be the same for
> yeast. See Figure D1 toward bottom of page.
> http://www.carolina.com/biotech/kruezer&massey/methods.asp
>
> cheers
> rb
>
> *** Posted via a free Usenet account from http://www.teranews.com ***
Thanks for the link the instructions and pics there were very useful
and ill be checking out the pamphlet from the previous post too thanks
Cheers!
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Date: 31 May 2006 11:59:37
From: rb
Subject: Re: yeast culturing "Streaking"
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nickstrachan wrote:
> Does anyone have a link to pictures of yeast culturing partuicularly
> the streaking method on petri dishes or plates....Right now im reading
> an article upon the subject but i cant seem to picture it....
>
>> Stab the loop in clean agar, flick it through the just-streaked area two or three times, and >streak a small area of fresh agar. When done, there should be a rectangular patch of clear >agar extending to the center of the plate, with the stab-marks at the bottom.
>
>
> It goes onto one more step but i cant get my mind around this
> process...Guess im more of a hands on show type..so if anyone had any
> pics of the process i'd be much obliged.
>
This works for bacteria, though the principle should be the same for
yeast. See Figure D1 toward bottom of page.
http://www.carolina.com/biotech/kruezer&massey/methods.asp
cheers
rb
*** Posted via a free Usenet account from http://www.teranews.com ***
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