| |
Main
Date: 01 Jul 2006 14:55:00
From: John Bleichert
Subject: frozen WL yeast vial - still ok?
|
Hello All
We've had a bit of flooding locally and I was without power for 3+
days. I pulled a vial of WL ale yeast from the (warming) fridge and
put it in the freezer hoping to hang on to it. The power kicked in
over night, the freezer kicked in, and now I have a frozen WL vial.
Is the vial still ok? I always make a starter anyways, but if it's
possibly b0rked I can pick up a new vial at the LHBS today.
Thanks - JB
-----------------------------------------------
John Bleichert syborg@earthlink.net
The heat from below can burn your eyes out!!
|
|
| |
Date: 01 Jul 2006 10:02:01
From: John Krehbiel
Subject: Re: frozen WL yeast vial - still ok?
|
> The power kicked in
> over night, the freezer kicked in, and now I have a frozen WL vial.
>
> Is the vial still ok?
Most likely dead. Best get a new vial IMHO.
John
|
| | |
Date: 01 Jul 2006 17:11:15
From: John Bleichert
Subject: Re: frozen WL yeast vial - still ok?
|
John Krehbiel <j_krehbiel@hotmail.com > wrote:
>
>> The power kicked in
>> over night, the freezer kicked in, and now I have a frozen WL vial.
>>
>> Is the vial still ok?
>
> Most likely dead. Best get a new vial IMHO.
>
> John
>
Sheesh I hope not. I just tried to get to the homebrew store and it's
still surrounded by standing water. Got within a 1/4 mile of it and it
seemed to be up high enough to not be overrun, but I can't be sure,
couldn't see it that clearly. Phone is dead, too :-(. Doc's Homebrew
in Binghamton, NY.
I hope Doc makes it.
( insert moment of silence )
In any event, I'm going to try that WL vial in a starter this afternoon..
-----------------------------------------------
John Bleichert syborg@earthlink.net
The heat from below can burn your eyes out!!
|
| |
Date: 01 Jul 2006 17:00:34
From: Todd
Subject: Re: frozen WL yeast vial - still ok?
|
Freezing will kill some of the Yeast, but some of them will survive.
Make a starter a couple of days earlier that you would normally. If it
takes off fine, if not, you will still have time to get another vial.
Todd
"John Bleichert" <syborg@earthlink.net > wrote in message
news:81wpg.1041$cd3.739@newsread3.news.pas.earthlink.net...
> Hello All
>
> We've had a bit of flooding locally and I was without power for 3+
> days. I pulled a vial of WL ale yeast from the (warming) fridge and
> put it in the freezer hoping to hang on to it. The power kicked in
> over night, the freezer kicked in, and now I have a frozen WL vial.
>
> Is the vial still ok? I always make a starter anyways, but if it's
> possibly b0rked I can pick up a new vial at the LHBS today.
>
> Thanks - JB
>
>
> -----------------------------------------------
> John Bleichert syborg@earthlink.net
> The heat from below can burn your eyes out!!
|
| |
Date: 02 Jul 2006 04:50:01
From: John 'Shaggy' Kolesar
Subject: Re: frozen WL yeast vial - still ok?
|
On Sat, 01 Jul 2006 14:55:00 GMT, <syborg@earthlink.net > wrote:
> Hello All
>
> We've had a bit of flooding locally and I was without power for 3+
> days. I pulled a vial of WL ale yeast from the (warming) fridge and
> put it in the freezer hoping to hang on to it. The power kicked in
> over night, the freezer kicked in, and now I have a frozen WL vial.
Freezing the yeast can kill them, it depends on how frozen the vial
got. IE, was it just slushy or was it a solid block of ice. When the
water that is absorbed inside the yeast cell freezes, it crystalizes and
expands, often rupturing the cell. The yeast doesn't tend to like that.
> Is the vial still ok? I always make a starter anyways, but if it's
> possibly b0rked I can pick up a new vial at the LHBS today.
IMO, I'd definitely make a starter first to make sure it's alright, but
there's a posibility that it's bOrked.
John.
|
| |
Date: 01 Jul 2006 19:01:48
From: Stoutman
Subject: Re: frozen WL yeast vial - still ok?
|
"John Bleichert" <syborg@earthlink.net > wrote in message
news:81wpg.1041$cd3.739@newsread3.news.pas.earthlink.net...
> Hello All
>
> We've had a bit of flooding locally and I was without power for 3+
> days. I pulled a vial of WL ale yeast from the (warming) fridge and
> put it in the freezer hoping to hang on to it. The power kicked in
> over night, the freezer kicked in, and now I have a frozen WL vial.
>
> Is the vial still ok? I always make a starter anyways, but if it's
> possibly b0rked I can pick up a new vial at the LHBS today.
>
> Thanks - JB
>
>
Sounds like a good time to get into yeast culturing. Streak them onto a
peatry dish and see if you can get any colonies to grow. If you do, you can
make a strter from just a few colonies.
If you don't have any petri dishes you can order them from www.morebeer.com.
http://morebeer.com/browse.html?category_id=1153&keyword=&x=1&y=1
For the media use gelatin and DME. Once cultured you can store them in
your fridge for 4-5 months before they get ugly, then all you have to do is
pluck off a colony and re-colonize a new petri dish.
I use to have 3-4 yeast strains on hand in petri dishes in the fridge. The
wife hated it, but...
|
| | |
Date: 01 Jul 2006 19:03:12
From: Stoutman
Subject: Re: frozen WL yeast vial - still ok?
|
Thats petri dish, not peatry dish. Oops.
|
| | |
Date: 01 Jul 2006 19:12:18
From: Stoutman
Subject: Re: frozen WL yeast vial - still ok?
|
Here is a REALLY good article on yeast culturing:
http://hbd.org/brewery/library/YeacultBL.html
|
|
