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Main
Date: 05 Jul 2006 22:09:33
From: Rob
Subject: Slants, Gelatin, and Nutrients
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I have been reading the faqs and threads on slants. I would like to
give slants a try. Initially I want to try Gelatin but it sounds like
Agar is preferred so I'll probably order some. However I'm confused
about nutrients. Can I use dry malt extract and standard nutrients for
beer, as well as wine yeast? If I shouldn't use DME for wine yeast
what can I use? Sugar or wort with nutrients?
Thanks, Rob
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Date: 06 Jul 2006 12:25:27
From:
Subject: Re: Slants, Gelatin, and Nutrients
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A slant tube has some nutrient in a medium which is solid at room
temperature (example: malt agar). You take a colony of yeast that you
know to be pure (example: a healthy colony from a petri dish or some
dregs from the bottom of a white labs vial) and smear it out on the
medium in the tube for later use.
I'm not sure what you mean by "nutrient". Are you saying you want to
make your own agar by somehow setting up a solution of dme so that it's
solid (like with gelatin)? I'm sure this can be done, but you'd have
to autoclave the medium to make sure it was absolutely sterile.
As an alternative, I recomend buying prefilled petri dishes and slants
from a brew shop (like morebeer.com). They're pretty cheap and easy to
use, and you can be fairly certain that they're clean.
-Nick
Rob wrote:
> I have been reading the faqs and threads on slants. I would like to
> give slants a try. Initially I want to try Gelatin but it sounds like
> Agar is preferred so I'll probably order some. However I'm confused
> about nutrients. Can I use dry malt extract and standard nutrients for
> beer, as well as wine yeast? If I shouldn't use DME for wine yeast
> what can I use? Sugar or wort with nutrients?
>
> Thanks, Rob
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Date: 11 Jul 2006 07:19:34
From: CarlJF
Subject: Re: Slants, Gelatin, and Nutrients
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If I understand you well, you want to make slant of wine yeast. If so,
then DME isn't the best thing to use for wine yeast. DME, or maltose,
is added for beer yeast because you don't want this kind of yeast to
lose their ability to use maltose (which is one of the main sugars in
beer wort). There's not much, if any, maltose in wine wort, and thus
you don't need to use DME or maltose in wine yeast slant.
For wine yeast, the classical media for slant, based only on glucose or
fructose, is certainly a more approriate media.
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Date: 11 Jul 2006 20:25:26
From: sosman
Subject: Re: Slants, Gelatin, and Nutrients
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Rob wrote:
> I have been reading the faqs and threads on slants. I would like to
> give slants a try. Initially I want to try Gelatin but it sounds like
> Agar is preferred so I'll probably order some. However I'm confused
> about nutrients. Can I use dry malt extract and standard nutrients for
> beer, as well as wine yeast? If I shouldn't use DME for wine yeast
> what can I use? Sugar or wort with nutrients?
I make my own malt agar for slants. Its very simple. The full deal is
at http://brewiki.org/Yeast/Culturing
I have only used this for beer yeast, not wine.
--
brewiki: http://brewiki.org/
Brewsta cross platform, open source brewing software:
http://brewsta.sourceforge.net
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Date: 11 Jul 2006 11:03:13
From: Rob
Subject: Re: Slants, Gelatin, and Nutrients
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Yes you understood me correctly, and that was the info I needed thanks!
CarlJF wrote:
> If I understand you well, you want to make slant of wine yeast. If so,
> then DME isn't the best thing to use for wine yeast. DME, or maltose,
> is added for beer yeast because you don't want this kind of yeast to
> lose their ability to use maltose (which is one of the main sugars in
> beer wort). There's not much, if any, maltose in wine wort, and thus
> you don't need to use DME or maltose in wine yeast slant.
>
> For wine yeast, the classical media for slant, based only on glucose or
> fructose, is certainly a more approriate media.
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